This interview features: Jin Zhou, Co-Founder, Business Leader, Purilogics By Donaldson, from BioProcess International 2023.

Purilogics’ Jinxiang Zhou recently co-authored an article alongside Camila Leal-Lopes, Sara D’Angelo, Andre A.R. Teixeira, Andrew R.M Bradbury, Fortunato Ferrara, and M. Frank Erasmus – the Head of Bioinformatics at Specifica, Inc. We are excited to announce that this article has been published in the New Biotechnology Journal: High throughput purification of monoclonal recombinant antibodies using a Protein-A coated membrane plate system.

Gene therapy involves the manipulation of gene expression pathways in cells utilized in the treatment of both genetic and pathological diseases. The delivery of genetic-based material, such as RNA or DNA, to cells modifies their expression patterns.

Recent developments in research have highlighted the potential of non-viral nanoparticles as vectors in gene therapy. Gene therapy typically refers to the use of nucleic acids, such as plasmids and siRNA, to genetically modify patient’s cells.1 Traditionally, viral vectors have been pursued as gene delivery vehicles, but a variety of risks and problems associated with them have limited their large-scale use clinically.

Recent developments have made small-scale antibody expression much easier. Upstream scientists can express tens or hundreds of antibodies in parallel. Analysis of purified samples at early stage will likely improve long-term success rate because there will be a reduction in interference of impurities.

In vitro-transcribed (IVT) mRNA is an alternative genetic material that can be used in the replacement of plasmid DNA in conventional uses…

How can Purilogics help? We offer membrane columns for small-scale antibody purification. Our mini and midi columns can be used with a syringe to purify small volumes of antibody.

Over the past few years, we have seen a steady increase in the demand for plasmid DNA, as the gene and cell therapy industries have experienced rapid growth…

Hydrophobic interaction chromatography (HIC) separates molecules based on differences in their hydrophobicity. It is an important technique in both sample analysis and preparative scale purification…

mRNA impurities need to be removed to achieve high potency and safety throughout the production process. mRNA production begins with plasmid DNA, which then needs to be linearized.

Purilogics offers a wide variety of differentiated products for your purification needs. Here are some highlights on the uniqueness of our membrane chromatography products:..

Fast protein liquid chromatography (FPLC) is a common purification technique used widely in various biologics preparation. Often, we complain how slow chromatography columns are, but we have ignored that FPLC itself can cause more trouble than it is worth.

Over the past 18 months, there has been a sharp rise in interest concerning the development of mRNA-based therapies, as highlighted by the FDA’s Emergency Use Authorization and subsequent approval of one of two novel SARS-CoV-2 mRNA vaccines.

A key focus of Purilogics is to emphasize the drastic decrease in processing time when using our membranes for biologics purification…

Imagine a glass holding sugar. After adding water, it is left to sit. Ultimately, the sugar will dissolve after a few hours…

At Purilogics, we take pride in designing and developing membrane chromatography technologies for biologics purification. Our mission is to provide rapid and effective purification solutions to help the biopharmaceutical industry simplify biologics purification.

mRNA contains specific triplets of nucleobases (codons) that encode for different amino acids for the ribosome to follow and stitch together a peptide chain that will eventually (after some modification) become a functional protein…

Antibody purification often involves the use of resin chromatogra¬phy columns for capture and polishing step purifications. However, pore diffusion of proteins is severely hindered in resins, resulting in low, flow rate-dependent capacity.

It is quite normal to see low titer recombinant protein feedstreams, such as Fc fusion protein, especially during early stage drug research and discovery. To obtain enough purified recombinant proteins, a large volume of feedstream is usually needed.

Magnetic beads are generally effective to purify antibodies at small scale, but there are quite a few scenarios that they become a detriment to your workflow, such as:…