Purexa™ NAEX Plus
Chromatography Membrane
Purexa™ NAEX Plus
for Supercoiled pDNA Capture
A novel weak anion-exchange membrane chromatography product, Purexa™ NAEX Plus has high binding capacity with targeted molecules. For pDNA, Purexa™ NAEX Plus has a high conductivity and impressive recovery during the purification process while successfully removing unwanted impurities. Download Purexa™ NAEX Plus datasheet PDF →
Learn more about Purexa MCP.
How Purexa™ NAEX Plus Works
The proprietary structure of tertiary amine ligands on the NAEX Plus membrane binds plasmid DNA at selected buffer conditions, and uses high conductivity conditions to release the DNA. Purexa™ NAEX Plus offers consistency across flow rates and multiple cycles while offering:
+ Fast cycle times
+ Easy setup and breakdown
+ High throughput pDNA purification
+ Can be adapted for purifying viral vectors, vaccines, DNA, and proteins
Consistent Cycle Performance
(Buffer Condition:1M NaOH + 2M NaCl and the Testing Agent:0.1 mg/mL salmon sperm DNA)
Purexa™ NAEX Plus can be reused over multiple cycles.
Purexa™ NAEX Plus Products
We are currently offering the following form factors and volumes [below]. These products are available now for purchase on Shop or by contacting us at bioconsumables@donaldson.com
Larger scale and GMP ready products will be available soon.
Consistent performance
across flow rates…
Figure 1. DBC and Recovery data were collected by utilizing Purexa™ NAEX Plus Maxi with columns at multiple flow rates, 11kbp pDNA ran with K Acetate as loading buffer.
Consistent performance across plasmid DNA sizes…
Figure 2. pDNA sizes were tested with Purexa™ NAEX Plus Maxi with K Acetate loading buffer.
Consistent performance across multiple cycles.
Figure 3. A Purexa™ NAEX Plus Maxi column was reused five times with a proper CIP cycle between runs to show consistent pDNA binding.
Various Form Factors & Volumes
Purilogics, a Donaldson brand, offers a broad portfolio of Purexa™ membrane series products in various form factors like columns, well plates and cassettes, with membrane volumes as small as 5.5 microliters up to several liters, as well as custom purification solutions.
Purilogics’ membrane chromatography products can be applied for the purification of many sizes of biologics while also maintaining and a rapid flow rate and high binding capacity.
Articles related to NAEX Plus will populate here. I’ll reroute it to “latest news” if we launch without NAEX specific articles
