In this presentation, Purilogics by Donaldson introduces the concept of using high-performance membrane chromatography technologies to rapidly purify SC pDNA and mRNA. We will illustrate purifying SC pDNA by capture chromatography using Purexa™ NAEX Plus, a weak anion chromatography membrane, followed by a polishing step using Purexa™ MCP, a hydrophobic interaction chromatography membrane that has superior and consistent capability to separate SC pDNA from open circular pDNA. We will illustrate purifying mRNA using Purexa™ OdT, an affinity capture chromatography membrane targeting the Poly A tails in mRNA.

mRNA impurities need to be removed to achieve high potency and safety throughout the production process. mRNA production begins with plasmid DNA, which then needs to be linearized.

mRNA contains specific triplets of nucleobases (codons) that encode for different amino acids for the ribosome to follow and stitch together a peptide chain that will eventually (after some modification) become a functional protein…